Regarding FMR transcription by FXS HESCs we show that FMR

2018-10-20

Regarding FMR1 transcription by FXS HESCs, we show that FMR1 expression in unmethylated FXS HESCs (HEFX, 0% methylation) is indistinguishable from WT HESCs and is significantly reduced in all methylated FXS cell lines. Nevertheless, the relation between the extent of methylation and FMR1 expression

iPSC derived iHLCs offer a unique

2018-10-20

iPSC-derived iHLCs offer a unique advantage in that they can be generated from any donor, which allows a broad spectrum of the human population to be represented in in vitro drug screens and provides an opportunity to assess donor-specific drug responses in vitro. Recent collaborations between drug

br Experimental Procedures Extended details outlining specif

2018-10-20

Experimental Procedures Extended details outlining specific steps and protocols can be found in the Supplemental Experimental Procedures. Author Contributions Acknowledgments Introduction Proximal spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder caused by t

br Materials and methods br Resource table

2018-10-20

Materials and methods Resource table Material and methods Introduction While increasingly effective for treating childhood cancers, chemotherapeutic agents and radiotherapy are known to have deleterious effects on the gonads of prepubertal boys (Schrader et al., 2001; Wallace, 2011). As

br Materials and methods br

2018-10-20

Materials and methods Results Discussion NPY is one of the most common neuropeptides in mammals, and it is abundant in the central and peripheral nervous system. Recent studies have indicated that the neuropeptides SP and NPY can regulate many biological activities in BMSCs and MC3T3 MLN 97

br Materials and methods br Acknowledgments This work was

2018-10-20

Materials and methods Acknowledgments This work was supported by Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (grant numbers SAF2013-43005-R and SAF2016-76004-R). The authors thank INDEPF (Instituto de investigación y desarrollo social de enfermedades

Molecular characterization of neurodegenerative pathologies

2018-10-20

Molecular characterization of neurodegenerative pathologies designates that reactive astrogliosis involves a number of changes in morphology, gene expression, and function of astrocytes, which in that state share hallmarks with NPCs (Robel et al., 2011). Astrocytes constitute the most abundant glial

The genomic stability of hIPSCs has been

2018-10-20

The genomic stability of hIPSCs has been extensively studied since this could be a major limitation for clinical applications (Aasen et al., 2008). Factors that affect the genomic integrity of stem rxr receptor include culture conditions, number of passages, and enzymatic and non-enzymatic dissociat

Small or immature teratomas are particularly

2018-10-20

Small or immature teratomas are particularly hard to detect due to the lack of tissues with high secretion levels such as gland-like structures. In line with that, we found one teratoma in our test set for which none of the eight tested biomarkers was positive. Histological characterization showed t

br Introduction Cardiovascular diseases rank the first in

2018-10-20

Introduction Cardiovascular diseases rank the first in cause of death, which account for ∼30% of all deaths worldwide (Mozaffarian et al., 2015). Unlike robust cardiac regeneration observed in adult zebrafish and embryonic and neonatal mice (Porrello et al., 2011; Poss et al., 2002; Sturzu et al.

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2018-07-29